Fabrizio santino biography samples
| Prince is 45 years old at the start of the novel and is married to Princess Maria Stella, with whom he has seven children. The son of a German princess, he has an authoritarian and morally rigid streak and is more inclined to abstract thought than to pragmatism. His main hobby, for instance, is astronomy; he has even discovered two small planets. The Prince also prefers other solitary pursuits like hunting. Both these hobbies--focused on the stars and the ancient Sicilian wilderness, respectively--allow the Prince to maintain the illusion that his world is stable. | |
| Tancredi, the Prince of Falconeri, is the Prince of Salina's nephew. He's the son of the Prince's sister and a spendthrift father who died when Tancredi was 14. Tancredi is now the Prince's ward, and the Prince loves him like a son, even wishing that Tancredi could be his heir. Tancredi has a lively and fun-loving personality marked by occasional seriousness. By the time of the Prince's death in 1888, Tancredi has built a successful political career. After Tancredi's own death, Concetta learns that Tancredi always loved her. | |
| Father Pirrone is a Jesuit priest, the Salina family chaplain, and the Prince's friend. He disapproves of the new liberal politics that are becoming popular in Sicily--especially because he believes that the Italian State will seize the Catholic Church's properties, thus disrupting the Church's traditional role as benefactor to Sicily's poor. Father Pirrone is portrayed as unfailingly faithful to the Salinas, as well as a sincere and devout Catholic. Father Pirrone comes from a humble peasant village, and his outsider perspective gives him unique insight into the character of the noble class. He believes that no matter what happens politically, the nobility will always renew itself in different forms, because nobility has more to do with attitude than blood. | |
Concetta is the Prince and Princess's second-oldest daughter and the Prince's favorite of the List of EastEnders characters introduced in 2023EastEnders is a BBC soap opera that first aired on 19 February 1985. The following is a list of characters that first appeared in 2023, in order of first appearance. All characters are introduced by the show's executive producer, Chris Clenshaw. The first character to be introduced is Brett Nelson (Fabrizio Santino), a man from Zack Hudson's (James Farrar) past; he appears in January. Emma Harding (Patsy Kensit), the estranged mother of Lola Pearce (Danielle Harold), and Jed (Bradley Jaden), a lodger for Sonia Fowler (Natalie Cassidy), also first appear in January. Jo Cotton (Vicki Michelle), the estranged wife of Tom "Rocky" Cotton (Brian Conley), and guest characters Shiv (Peter Caulfield) and Troy (Chris Evangelou) debut in March. Theo Hawthorne (William Ellis), the former teacher of Freddie Slater (Bobby Brazier), first appears in April. The Knight family – consisting of patriarch George Knight (Colin Salmon), his two daughters Gina Knight (Francesca Henry) and Anna Knight (Molly Rainford), and Tyson, their pet Chihuahua – are introduced in June. Jasper Parrott, Rocky's pet parrot, and DS Miles (Samantha Seager), a detective sergeant investigating Suki Panesar's (Balvinder Sopal) fall, also debut in June. Caz Johnston (Bryony Afferson), a woman from Eve Unwin's (Heather Peace) backstory, appears in August. September features the introductions of Charli Slater, the newborn daughter of Lily Slater (Lillia Turner) and Ricky Branning (Frankie Day), and Nadine Keller (Jazzy Phoenix), a love interest for Jay Brown (Jamie Borthwick). The following month, Priya Nandra-Hart (Sophie Khan Levy), the former partner of Ravi Gulati (Aaron Thiara), and their daughter, Avani Nandra-Hart (Aaliyah James), joined the cast. Pastor Clayton (Howard Saddler) made his first appearance in December. Additionally, multiple other characters were featured during the year. Brett NelsonBrett Nelson, The energetic metabolism of cancer cells relies on a substantial commitment of pyruvate to the catalytic action of lactate‐generating dehydrogenases. This coupling mainly depends on lactate dehydrogenase A (LDH‐A), which is overexpressed in different types of cancers, and therefore represents an appealing therapeutic target. Taking into account that the activity of LDHs is exclusively exerted by their tetrameric forms, it was recently shown that peptides perturbing the monomers‐to‐tetramer assembly inhibit human LDH‐A (hLDH‐A). However, to identify these peptides, tetrameric hLDH‐A was transiently exposed to strongly acidic conditions inducing its dissociation into monomers, which were tested as a target for peptides at low pH. Nevertheless, the availability of native monomeric hLDH‐A would allow performing similar screenings under physiological conditions. Here we report on the unprecedented isolation of recombinant monomeric hLDH‐A at neutral pH, and on its use to identify peptides inhibiting the assembly of the tetrameric enzyme. Remarkably, the GQNGISDL octapeptide, mimicking the 296–303 portion of hLDH‐A C‐terminal region, was observed to effectively inhibit the target enzyme. Moreover, by dissecting the action of this octapeptide, the cGQND cyclic tetrapeptide was found to act as the parental compound. Furthermore, we performed assays using MCF7 and BxPC3 cultured cells, exclusively expressing hLDH‐A and hLDH‐B, respectively. By means of these assays we detected a selective action of linear and cyclic GQND tetrapeptides, inhibiting lactate secretion in MCF7 cells only. Overall, our observations suggest that peptides mimicking the C‐terminal region of hLDH‐A effectively interfere with protein–protein interactions responsible for the assembly of the tetrameric enzyme. Keywords: human lactate dehydrogenase A, inhibition of assembly, monomer, peptides, subunit–subunit interaction Lactate dehydrogenases are stereospecific enzymes, . | |