Saurin bhatt biography template

Ahumada A.V., Claria J.J., Bica E., Piatti A.E.
(2000) Astron. Astrophys. Suppl. 141, 79
Integrated spectral properties of 7 galactic open clusters
2000A&AS..141...79A

Anthony-Twarog B.J., Twarog B.A.
(2000) Astron. J. 119, 2282
CCD uvby Hbeta photometry in clusters. I. The open cluster standard, IC 4651
2000AJ....119.2282A

Debernardi Y., Mermilliod J.-C., Carquillat J.-M., Ginestet N.
(2000) Astron. Astrophys. 354, 881
Binarity of Am stars in Praesepe and Hyades
2000A&A...354..881D

Delgado A.J., Alfaro E.J.
(2000) Astron. J. 119, 1848
Search for pre-main-sequence stars in the young galactic cluster NGC 6910
2000AJ....119.1848D

Dutra C.M., Bica E.
(2000) Astron. Astrophys. 359, 347
Foreground and background dust in star cluster directions

Anatomy

Karla Coburn, DDS, PhD

Instructor of Anatomy

Darin Croft, PhD

Professor of Anatomy

Andrew Crofton, PhD

Assistant Professor of Anatomy

Clifford Harding, MD, PhD

Interim Chair and Professor of Anatomy

Meghan Newcomer, PhD

Instructor of Anatomy

Scott Simpson, PhD

Professor of Anatomy

Susanne Wish-Baratz, PhD, MBA

Associate Professor of Anatomy

Anesthesiology

Mohamed Abdalla, MD

Clinical Assistant Professor of Anesthesiology

Hesham Abdelaziz Elsharkawy, MBBS

Associate Professor of Anesthesiology

Basem Abdelmalak, MD

Professor of Anesthesiology

Joseph Abdelmalak, MBBS

Clinical Associate Professor of Anesthesiology

Bushra Abdul Aleem

Assistant Professor of Anesthesiology

Ira Abels, MD

Clinical Assistant Professor of Anesthesiology

Hinda Abramoff, MD

Assistant Professor of Anesthesiology

Ahmad Adi, MD

Clinical Assistant Professor of Anesthesiology

Avneep Aggarwal, MBBS

Clinical Assistant Professor of Anesthesiology

Benigno Aldana, MD

Clinical Assistant Professor of Anesthesiology

Andrej Alfirevic, MD

Professor of Anesthesiology

Ali Ali, DO

Assistant Professor of Anesthesiology

Jonathan Alter, MD

Assistant Professor of Anesthesiology

Balaram Anandamurthy, MBBS

Clinical Assistant Professor of Anesthesiology

C. Angela Cesar, MD

Assistant Professor Emeritus of Anesthesiology

David Anthony, MD

Clinical Assistant Professor of Anesthesiology

John Apostolakis, MD

Clinical Assistant Professor of Anesthesiology

Maged Argalious, MBBS

Professor of Anesthesiology

Sherif Assaad, MBBch

Associate Professor of Anesthesiology

Brendan Astley, MD

Assistant Professor of Anesthesiology

Rafi Avitsian, MD

Professor of Anesthesiology

Sabry Ayad, MD

Professor of Anesthesiology

Rafik Banoub, MBBS

Clinical Assistant Professor of Anesthesiology

Sabri Barsoum, MBBS

Assistant Professor of Anesthesiology

Charles Bashour, MD

Associate Professor of Anesthesiology

Hersimren Basi, MD

Clinical Assistan

Abstract

Uncontrolled resection of replication forks under stress can cause genomic instability and influence cancer formation. Extensive fork resection has also been implicated in the chemosensitivity of “BReast CAncer gene” BRCA-deficient cancers. However, how fork resection is controlled in different genetic contexts and how it affects chromosomal stability and cell survival remains incompletely understood. Here, we report a novel function of the transcription repressor ZKSCAN3 in fork protection and chromosomal stability maintenance under replication stress. We show disruption of ZKSCAN3 function causes excessive resection of replication forks by the exonuclease Exo1 and homologous DNA recombination/repair protein Mre11 following fork reversal. Interestingly, in BRCA1-deficient cells, we found ZKSCAN3 actually promotes fork resection upon replication stress. We demonstrate these anti- and pro-resection roles of ZKSCAN3, consisting of a SCAN box, Kruppel-associated box, and zinc finger domain, are mediated by its SCAN box domain and do not require the Kruppel-associated box or zinc finger domains, suggesting that the transcriptional function of ZKSCAN3 is not involved. Furthermore, despite the severe impact on fork structure and chromosomal stability, depletion of ZKSCAN3 did not affect the short-term survival of BRCA1-proficient or BRCA1-deficient cells after treatment with cancer drugs hydroxyurea, PARPi, or cisplatin. Our findings reveal a unique relationship between ZKSCAN3 and BRCA1 in fork protection and add to our understanding of the relationships between replication fork protection, chromosomal instability, and chemosensitivity.

Keywords: replication stress, fork resection, ZKSCAN3, BRCA1, chemosensitivity

Abbreviations: CldU, chlorodeoxyuridine; DSB, double-strand DNA break; HR, homologous recombination; HU, hydroxyurea; IdU, iododeoxyuridine; PARPi, PARP inhibitors; PBS, phosphate-buffered saline; PBST, Triton X-100 in PBS; ZNF, zinc finger

Abstract

Novel proteins can originate de novo from non-coding DNA and contribute to species-specific adaptations. It is challenging to conceive how de novo emerging proteins may integrate pre-existing cellular systems to bring about beneficial traits, given that their sequences are previously unseen by the cell. To address this apparent paradox, we investigated 26 de novo emerging proteins previously associated with growth benefits in yeast. Microscopy revealed that these beneficial emerging proteins preferentially localize to the endoplasmic reticulum (ER). Sequence and structure analyses uncovered a common protein organization among all ER-localizing beneficial emerging proteins, characterized by a short hydrophobic C-terminus immediately preceded by a transmembrane domain. Using genetic and biochemical approaches, we showed that ER localization of beneficial emerging proteins requires the GET and SND pathways, both of which are evolutionarily conserved and known to recognize transmembrane domains to promote post-translational ER insertion. The abundance of ER-localizing beneficial emerging proteins was regulated by conserved proteasome- and vacuole-dependent processes, through mechanisms that appear to be facilitated by the emerging proteins’ C-termini. Consequently, we propose that evolutionarily conserved pathways can convergently govern the cellular processing of de novo emerging proteins with unique sequences, likely owing to common underlying protein organization patterns.

Keywords:de novo gene birth, de novo proteins, ER, targeting, degradation, localization

Introduction

New protein-coding genes can evolve de novo from sequences that were previously non-genic (Fig 1A). Once considered rare, de novo gene birth has now been identified in many species and is gaining considerable attention as a mechanism of molecular innovation and species-specific adaptation (Van Oss and Carvunis 2019, Weisman 2022, Broeils, Ruiz-Orera et al. 2023, Zha